The sponge like protocol successfully introduced a fast, cheap and in house cell evacuation for nearly all the biological cells . The original protocol describes bacterial cell evacuation from its cytoplasmic contents [1,2]. The protocol was optimized using Plackett-Burman design, which enables randomization for the variables included. Such randomization enables mapping the best cells’ evacuation experimental conditions, which give the best un-deformed 3D structure and un-deteriorated surface antigens [1,3]. Its main concept is to determine the minimum inhibition concentration (MIC) and the minimum growth concentration (MGC) of chemical compounds able to induce pores in the cell walls [1,3].
Al-Fattah Amara AA
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